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Isolation of gluten from starch

  1. and starch granules. The procedure of wheat gluten preparation was first described by Beccari in 1745 and is nowadays widely used for the industrial isolation of vital gluten, a byproduct of wheat starch production. Gluten is a protein fraction from wheat, maida, barley, oats or their crossbred varieties and derivatives thereof, to whic
  2. Pulp obtained after isolation of starch from red and purple potatoes (Solanum tuberosum L.) as an innovative ingredient in the production of gluten-free bread Starch based gluten-free bread (formulations containing mixture of corn and potato starch with hydrocolloids) are deficient in nutrients and do not contain health promoting compounds
  3. The procedure of wheat gluten preparation was first described by Beccari in 1745 and is nowadays widely used for the industrial isolation of vital gluten, a byproduct of wheat starch production

starch plus fine fiber, the B-starch plus gluten, and the solubles plus finely suspended solids (Fig. 4). Removal of the viscous pentosans in the solubles fraction simplifies the isolation of gluten from the middle fraction of the decanter. In the HD process, a sheared slurry i In a process utilizing a battery of hydrocyclones for separating wheat starch and gluten contained in an aqueous wheat flour slurry, the slurry is supplied to a first section of the hydrocyclone.. Gluten can be readily prepared from wheat by washing dough to remove the bulk of the starch, cell-wall material, and soluble components. It is a cohesive viscoelastic mass that contains, in addition to the gluten proteins, small amounts of other proteins, residual starch (about 25% dry wt), and lipid Two different commercial bread wheat flours (BF‐I, 65% extraction and BF‐V, 86% extraction) were separated into gluten and starch milk by making a dough, allowing some time for maturation, dispersing the dough in water and wet sieving/washing sufficient to keep the starch soft and the gluten elastic) Below is a series of scanning electron micrographs (SEMs) of different stages of gluten development reproduced from the German journal Zeitschrift fur Lebensmittel-Untersuchung und-Forschung (1990) 190: 401-409. These photo

Fig. 1 shows the scheme of the extraction proce­ dure, used to separate starch and protein from the insoluble cell wall material of flours [18] which was modified for the isolation of the simultaneously dis­ solved polysaccharides. Due to the mild alkaline condi­ tions used, the release of easy accessible, water-soluble A. BURISOVÁ. B Gluten may be defined as the 'cohesive, visco-elastic proteinaceous material prepared as a by-product obtained by isolation of starch from wheat flour. A biological definition might include the origins of the gluten-protein complex as being derived from the 'storage proteins of the wheat grain' (Shewry and Halford 2002) The principle involved in the isolation of gluten is difference in solubility. The starch is partially soluble in water while gluten is insoluble in water. Thus, gluten can be separated from starch. Iodine solution is used to test the complete removal of starch, which involves the formation of blue-iodostarch complex) The old system of gluten separation in the wheat starch process, called the Martin Process, has been progressively replaced by the modern three-phase process based on the Tricanter®. Flottweg has accompanied this development from the very beginning and has worked with their customers to optimize the processes

It was successfully applied for the quantification of volatiles in corn starch and qualitative comparison of different gluten-free flours. Hexanal, 3-methyl-1-butanol, 1-pentanol, 1-octen-3-ol, acetic acid, furfural, benzaldehyde, (E)-2-nonenal, phenylethyl alcohol and short-medium chain acids were found in all the flours and corn starch 4-starch and gluten separation, starch washing The 4th step of the corn starch production process is starch and gluten separation from the starch milk which is obtained from the previous step. In this process, the starch left leaves the separator as the under flow whereas, on the other hand, the gluten leaves the separator as over flow

Worldwide, gluten is an important source of nutritional protein, both in foods prepared directly from sources containing it, and as an additive to foods otherwise low in protein Gluten is still extracted from flour by washing out the starch by means not fundamentally different from the ancient way, which exploited the fact that starch is water. Study material consisted of gluten-free bread with a share of 5; 7.5 and 10% freeze-dried potato pulp obtained after laboratory extraction of starch from potatoes with red (Magenta Love) and purple (Violetta) flesh. Potato pulp was obtained as a by-product in laboratory isolation of potato starch, according to Wischmann et al A nontechnical definition of gluten describes it as the sticky, viscous residue after removal of starch from flour. This definition would include corn gluten, the protein residue from isolation of starch from corn. However, this material is quite different to wheat gluten, the residue from production of wheat starch from flour Extraction with 1% PVP K15 successfully recovered gluten in cacao products. Abstract Enzyme-linked immunosorbent assay (ELISA) is commonly used to detect trace gluten in foods; however, ELISA for the determination of gluten is affected by matrices from various ingredients, such as plant proanthocyanidins (PACs)

From Wheat to Starch and Gluten in a Single Step When starch is produced, wheat flour gets broken down into its components: A-starch, pentosane and gluten, or B-starch. Using the decanter centrifuge, all of these components can be extracted in a closed system The starch and other constituents dissolve, but the gluten, which is not water soluble, does not. Once starch and gluten are separated by centrifugation, the gluten is washed thoroughly and dried. The temperature never exceeds 70 degrees Celsius, in order to protect the gluten's functional properties, known as vitality

The starch is thus gradually washed off from the elastic gluten whereby both the starch and the gluten eventually are separated from each other in very pure fractions. The method however requires the use of large quantities of water, generally 10-17 fold water per quantity of flour. Additionally, usually about 8-10, and even up to 20%, of the. A laboratory‐scale process is presented for the manufacture of starch and gluten from wheat. Main feature of this process is that whole wheat kernels are crushed dry between smooth rolls prior to wet disintegration in excess water in such way that gluten formation is prevented and fibres can be removed by sieving The principle involved in the isolation of gluten is the difference in solubility. The starch is soluble in water while gluten is insoluble thus gluten can be separated from starch. Iodine solution is used to test the complete removal of starch giving no blue-violet coloration on the washings once it is free from unwanted starch The main components of wheat are fiber, starch and gluten protein. Extraction involves milling the wheat into flour, making dough and washing out the starch. Starch dissolves in water but gluten does not, so the gluten-protein sediment sinks to the bottom. Then the starch solution can be drained off and dried gluten. Goals / Objectives. 1. To determine the commercial feasibility of winter waxy (amylose-free) wheats produced in Great Plains environments. 2. To identify commercial applications for amylose-free starch isolated from pilot-scale extractions of waxy wheat grain. 3. To determine the efficiency of gluten extraction from waxy wheats

Pulp obtained after isolation of starch from red and

after the extraction of the larger portion of the starch, gluten, and germ by the processes employed in the wet milling manufacture of corn starch or syrup. It may or may not contain one or both of the following: fermented corn extractives, corn germ meal. (Adopted 1936, Amended 1960.) IFN 5-02-903 Maize gluten meal The controversy over the use of wheat starch in gluten-free foods is back on the front burner due to the new DiGiorno gluten-free pizzas. The pizzas are made using wheat starch that has been processed to remove gluten. Before we go any further, please remember that under the FDA's 2013 gluten-free labeling rule, wheat starch [ Based on the isolation of gluten by washing a flour/water mixture; Some mills and bakeries around the world rely on the percent of wet gluten and gluten index as important flour specifications. Gluten washing is simply the process of using excess water to mechanically remove the starch and most other non-gluten components of the wheat flour

Extraction of gluten from food material - MedCrave onlin

Starch Isolation from Selected Wheat Varieties as Affected by Nitrogen Fertilization Intensity and the Relation to Protein Content and Gluten Composition Federal Centre for Nutrition and Food, Institute for Cereal, Potato and Starch Technology, Detmold, Germany 58 Starch Convention Detmold 25 Œ 27 April 200 ISOLATION AND CHARACTERIZATION OF GLUTEN FROM WHEAT FLOUR Joe Renz N. Joya, Patrick Angelo D. Juacalla, Katherine Joy C. Kalalo, Donna Marie S. Ledesma and Nenet V. Licuanan Group 6 2F-Pharmacy Biochemistry Laboratory ABSTRACT Gluten was the protein found in wheat which remained when wheat dough was washed to take away starch granules and water-soluble constituents Rinse the starch (and chaff, if you stared with whole wheat flour) away from the gluten with water. Using about 10 mL of water (two teaspoons) for each wash has worked in the past. You can do the wash step in the bag, carefully mixing the dough with the water, and pouring out the liquid When starch is produced, wheat flour gets broken down into its components: A-starch, pentosane and gluten, or B-starch. Using the decanter centrifuge, all of these components can be extracted in a closed system. The Flottweg Tricanter ® is the world's most efficient method for wheat starch extraction. It separates the contents in one step after 5 minutes' rotation and finally, addition of sediment starch to petri-dish are sequential activates done during gluten separation (Figure 2). Figure 2: Centrifugal Starch separation process. Figure 1: Starch extraction process from butula lenta bark

The starch-gluten suspension, called mill starch, is piped to the starch separators. Starch Separation Gluten has a low density compared to starch. By passing mill starch through a centrifuge, the gluten is readily spun out for use in animal feeds. The starch, with just one or two percen of the starch, cell-wall material, and soluble components. It is a cohesive vis- Extraction of Wheat Gluten and Related Proteins 57 for detailed characterization, or clinical challenges. It is. Potato starch doesn't differ much from gluten starches when it comes to nutrition. One ounce of potato starch has 100 calories, 23.3 grams of carbs and 1.9 grams of protein; the most common gluten thickener, all-purpose flour, has 102 calories, 21.4 grams of carbs and 2.9 grams of protein

Wheat Starch: Isolation, Structure and Propertie

SOg which ia combined in gluten water . 110-^ 6, Benzene Extraction of SOo from Starch IVash Water 113 7, Benzene Extraction of SOg froni Starch Wash Water Acidified with HCl 114 a. Effect of excess HCl 114 b. Amount of 10% HGl necessary to liberate combined SO2 in starch wash water . . . 114 DISCUSSION Al® RESULTS . . 11 Rasping machine can improve the crushing and extraction rate. Step 3 Screening. Centrifuge sieve is mainly used to separate the fiber and starch slurry. The sieve basket is made of titanium plate, with laser punching processing. In the plant of isolation of starch from potato, the machine is necessary for the high quality final product Worldwide consumption of wheat, estimated by World Gluten may be defined as the 'cohesive, visco- Agricultural Supply and Demand Estimates elastic proteinaceous material prepared as a (WASDE), has been found to be by-product of the isolation of starch from 652.18 million ton for year 2010 [2] In corn wet milling process, after extraction of germ and fiber, centrifugal separators are normally used for separation of gluten from starch, yielding thin gluten liquid of 15-25g/l density.

But wet gluten is not just water and protein; it also contains about 5 - 10% lipids (d.b.) and a significant amount of non-starch carbohydrates (Pomeranz, 1988). It is clear that the functionality of extracted and dried gluten that is re-added to flour will differ from the gluten naturally present Increased awareness and diagnosis of CD and gluten sensitivity have spurred the demand for gluten-free products. Mintel (2007) reported that the gluten-free foods and beverages market in 2006 was $700 million and is projected to grow annually at a rate of 15% to 25% to $1.3 billion by 2010. The largest numbers o Isolated wheat starch is often utilised in gluten-free products, but starch-based ingredients should ideally originate from raw materials that are naturally gluten-free . Different starches from naturally gluten-free sources such as corn, cassava, potato and rice have been utilised in gluten-free formulations [12,13,14,15]

US4494530A - Separation of gluten and starch from wheat

  1. Keywords: Starch isolation; Protein; Gluten; Oat 1 Introduction Starch can be separated from wheat by a number of industrial processes, but is difficult to separate from nonwheat sources. One of the oldest methods for isolating wheat starch is a modified version of the Martin method that has been in use since 1835 [1]
  2. Wheat gluten is obtained by physical extraction when mixing wheat flour with water. The starch is washed out of the dough andthe gluten remains. Once separated and dried, wheat gluten is an insoluble concentrated protein powder, which regains its original characteristics after rehydration and mixing
  3. ation, fine grinding, gluten separation, starch refining, dehydradration, drying etc. Stepping contributes to being an essential process for the production of high quality starch. The kernels of the maize starch are being dipped into the water
  4. for breads baked with gluten-starch blends containing small or large starch granules. respectively. When coarse, medium, and fine starch granule preparations were used in baking experiments, no clear effects of the starch fractions on proof time, water absorp-tion, or bread volume were observed (Lelievre et al 1987). KuI
  5. starch and the water that contains other flour solubles pass through the screen. The suspension of starch in water is usually called starch milk. This starch milk, containing some fine fiber, soluble protein, and a small amount of fine gluten particles, may be used directly for fermenta- tion into industrial alcohol

Starch and pentosans are far more important to the quality of the dough yield than gluten. Starch is the chief component of the flour responsible for the structure of the loaf. Its bread-making ability hinges on the age of the flour and the acidity THE GLUTEN FRACTION is the complex middle phase. It contains the gluten, fibres, solubles, B-starch and some A-starch. After maturing of the gluten these constituents are split into sub-fractions. Gluten Maturing. Before separating the gluten fraction the stream is carefully treated in a maturing reactor Celiac disease is a gluten-induced hypersensitivity reaction that requires a lifelong gluten-free diet. Gluten-free foods must not contain more than 20 mg/kg gluten as laid down by Codex Alimentarius. Measuring the presence of gluten with routine immunoanalytical methods in food is a serious challenge as many factors affect accurate determination. Comparability of the results obtained with. starch, one of nature's fasci-nating architectural forms, is a vital element in the flex-ibility of commercial starches to fill specific prod-uct needs. Endosperm Germ Starch and Gluten Hull and Fiber Starch Corn kernels have three main parts: the seed coat or pericarp, the starchy en-dosperm, and the embryo, commonly called the germ (Figure 4.

Extraction, Separation, and Purification of Wheat Gluten

Separation of bread wheat flours into starch and gluten

  1. Manufacturer of Seitan extraction machine,Gluten extraction machine is a high efficient machine for gluten making.During the wheat starch production line,gluten extraction is the first important procedure.Gluten extraction machine is used for this process. +86 135 2661 5783
  2. Formal Biochem Lab Report -Isolation and Hydrolysis of Gluten - Free download as Word Doc (.doc), PDF File (.pdf), Text File (.txt) or read online for free. A formal lab report regarding the isolation and Hydrolysis of Gluten experimen
  3. The starch stream typically has 90% starch and the gluten stream consists of 60% protein. The lighter gluten, separated out from the top, is thickened and the heavy gluten is further sent for dewatering into vacuum rotary filter. This corn gluten meal consist of approximately 60% protein, 1% fat and 3% fiber
  4. C, Lysine, Citric acid, Antibiotic, Enzymic preparations, etc. 5.B-starch can be used for Food, feed and making ethyl alcohol

Isolation and Characterization of the Non-Starch

  1. Starch Gluten Stones. Solutions for processing starch 7 Alfa Laval and maize starch extraction With its acquisition of the Dorr-Oliver starch activities, Alfa Laval became the company with the largest installed base in the maize starch industry, with a vast body of know-how focused on all kind
  2. During this extraction process, potatoes are crushed, which releases starch grains, called leucoplasts. The starch grains then get washed and dried until a powder can be formed, which then becomes potato starch. It doesn't have a smell or taste, is soft white, and becomes a mostly clear, thick, gel-like paste. Potato starch is also gluten.
  3. Corn gluten meal is obtained wherever maize is used for starch extraction. It is distributed worldwide. Its production has become relatively constant since ethanol is now mainly produced by dry-milling, which yields corn distillers rather than corn gluten meal and corn gluten feed ().In 2010-2011, feed consumption of both corn gluten meal and corn gluten feed (statistics do not differentiate.
  4. or quantities of end-products from other microbial fermentations (Stock.
  5. Wheat Protein Concentrate - Isolation of wheat proteins starts with wet-processing of wheat flour where the protein component exists as a doughy, viscoelastic mass and the starch component exists as an aqueous dispersion. Passing through suitable screens or by using centrifugal action can successfully separate the protein from starch
  6. Corn Gluten Feed is the portion of the shelled corn that remains after extraction of the starch, gluten and germ by the wet-milling process at our Muscatine, Iowa, and Washington, Indiana, facilities and is produced in the following forms

The degerminated mill starch leaving the fine mill is pumped to the first stage of a fibre washing system, where starch and gluten is screened off. The overs, hull and larger fibres, are washed free from adhering starch and gluten (insoluble protein) on screens in counter-current with process wash water added at the last stage Meal: Like chop, meal is made of 100% extraction obtained by grinding the entire rye kernel. Light rye flour: This is obtained from the centre of the rye kernel and is low in protein and high in starch content. It can be compared to white bread flour and is used to make light rye breads Background . Starch and its products are used in a variety of ways for both the food and nonfood industries. A factorial experiment is carried out with two factors to explain the behavior of the percentage of starch, where the factors correspond to the extraction method and to the raw material. Method . Three methods were used in triplicate: the first followed the official technique of the. Hard flours are high in gluten, while soft flours are low. Blended from soft wheat varieties, cake flour typically contains only 7 percent to 9 percent gluten by weight. The all-purpose flour sold in most of the United States contains more hard wheat and can range from as low as 8 percent gluten to as high as 11 percent Tereos FKS Indonesia produces four types of glucose syrups for various applications. High Fructose Corn Syrup is a transparent to light yellow color syrup, obtained by the isomerization of glucose syrup. The relative sweetness of high fructose corn syrup is 1.2 to 1.6 times compared to sucrose (table sugar)

Isolation, characterization of wheat gluten and its

Alibaba.com offers 832 gluten extraction machine products. A wide variety of gluten extraction machine options are available to you, such as local service location, key selling points, and applicable industries Gluten may be defined as the 'cohesive, visco-elastic proteinaceous material prepared as a by-product obtained by isolation of starch from wheat flour. A biological definition might include the origins of the gluten-protein complex as being derived from the 'storage proteins of the wheat grain' (Shewry and Halford 2002 )

Proximate analysis of wheat starch after gluten extraction from Fd-08 variety (g/100g) Mean±SD values followed by different letter in a column are significantly different at p. 0.05 Data in Table 5 demonstrated that maximum starch was recovered in the dough batter and batter process. There was no gluten recovery in the chemical method Wheat Gluten, the major protein of wheat, has been defined as the cohesive, viscoelastic, proteinaceous material prepared as a by-product of the isolation of starch from wheat flour [32]. Gluten consists of gliadins and glutenins, which both contribute to the unique viscoelastic properties of wheat dough. Gliadin Starch plays a key role in gluten-free food production due to the fact that it is the main ingredient of gluten-free raw materials. Gluten-free food technology primarily relies on dough heating and cooling operations that exploit two phenomena: before starch gelatinization and, subsequently, its retrogradation A laboratory-scale process is presented for the manufacture of starch and gluten from wheat. Main feature of this process is that whole wheat kernels are crushed dry between smooth rolls prior to wet disintegration in excess water in such way that gluten formation is prevented and fibres can be removed by sieving. Centrifugation of the endosperm suspension yields a dough which can be separated.

Purified wheat starch (WSt) is commonly used in gluten-free products for celiac disease (CD) patients. It is mostly well-tolerated, but doubts about its safety for CD patients persist. One reason may be that most ELISA kits primarily recognize the alcohol-soluble gliadin fraction of gluten, but insufficiently target the alcohol-insoluble. gluten content of more than 5000 ppm and the micro-biological method reduced the gluten content from 2500 ppm to 398 ppm. From the results it was observed that no gluten extraction method is viable to produce starch which can fulfill the criteria of a gluten free pro-duct (20ppm). Key words: Wheat, gluten, extraction, starch, protein RESUMEN Refined starches are partially damaged during the processing and extraction, causing the starch to behave differently. While wheat-based goods are naturally high in starch, gluten-free foods have a higher concentration of refined starches because they rely on refined starches to replace the structure and binding properties that gluten provides. Making raw gluten from flour is a simple but step-wise process: Select the flour. Mix with water and rest. Knead to develop the proteins into gluten. Soak and rinse to remove starch and bran. Shape the raw gluten, with or without additions. Season and cook to flavor and firm the gluten in a cold simmering stock

Isolation and Characterization of Gluten from Wheat Flour

Starch is widely used as an ingredient and significantly contributes to texture, appearance, and overall acceptability of cereal based foods, playing an important role due to its ability to form a matrix, entrapping air bubbles. A detailed characterisation of five gluten-free starches (corn, wheat, rice, tapioca, potato) was performed in this study Two types of wheat flour C1 (high-content gluten) and C2 (low-content gluten) were used for gluten extraction, and the gluten was used to fabricate edible films. The dry and wet gluten content of C1 is higher than that of C2. The extraction solvents are water, water/ethanol (1:1, v/v), and 2% NaCl solution Following another centrifugation step, the starch residue was extracted once with 5 mL gluten extraction solution (2-propanol/phosphate buffer pH 7.6, 1+1, v/v, containing 10 mg/mL dithiothreitol under nitrogen) for 30 min at 60 °C in a water bath (→ glutenin extract)

Comparison of different extraction methodologies for the

Frontia ® GlutenEx is a premium enzymatic solution for the wheat flour separation industry. It improves the separation process and delivers very high recoveries of starch and gluten protein. Increased capacity utilization by rapid viscosity reduction and low fouling frequency of screen evaporator 2. The flour is mixed with water to form a dough and the starch and gluten are separated by a physical process. 3. The wet gluten is washed with water to remove residual starch and dried to wheat gluten. The wet gluten can be partially hydrolysed also to produce hydrolysed wheat gluten. 4. The starch slurry is washed with water and can be Alongside this, wheat starch is processed by eliminating the gluten proteins from wheat flour, which makes it a preferred choice among the consumers who prefer gluten-free foods Moist Corn Gluten is the part of commercial- shelled corn that remains after the extraction of starch, gluten and germ by processes used in the wet milling manufacture of corn syrup. The product includes the condensed corn extractives (steep), the corn ker nels hull and the process fibrous particles

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A brief description of the Corn Starch Production Process

Gluten extraction from deoxynivalenol contaminated wheat . Shopp ; The main components of wheat are fiber, starch and gluten protein. Extraction involves milling the wheat into flour, making dough and washing out the starch. Starch dissolves in water but gluten does not, so the gluten-protein sediment sinks to the bottom From the results it was observed that no gluten extraction method is viable to produce starch which can fulfill the criteria of a gluten free product (20ppm). Key words: Wheat, gluten, extraction, starch, protein. Eficacia comparativa de diferentes métodos de extracción de gluten en variedades autóctonas de trigo. RESUMEN. El presente roduc.

Extraction Of Gluten From Flour And Extraction Of Gliadin

Corn Gluten Feed. is a co-product of the corn wet-milling process and is the part of the corn kernel that remains after the extraction of the starch, germ, and gluten for production of corn starch or glucose. Application. Corn gluten feed is a feed ingredient mostly used in beef cattle diets as a source of energy and protei Wheat gluten is a food made from gluten, the main protein of wheat. It is made by washing wheat flour dough with water until all the starch granules have been removed, leaving the sticky insoluble gluten as an elastic mass which is then cooked before being eaten. The wheat Starch market is expected to exceed US$ 4 Billion by 2024 Starch Isolation High-intensity ultrasound (sonication) was used to purify starch from sorghum flour following the procedure of Park et al. (2006). The isolated starch was dried in a Labconco Freezone 6 Freeze Dryer (Labconco Corporation, Kansas City, MO). Starch Particle Size Distribution Starch particle size distribution was determined usin Gluten is a composite of the proteins gliadin and glutenin.These exist, conjoined with starch, in the endosperms of some grass-related grains, notably wheat, rye, and barley.Gliadin and glutenin comprise about 80% of the protein contained in wheat seed. Being insoluble in water, they can be purified by washing away the associated starch

Sorghum Proteins: The Concentration, Isolation

Corn gluten or corn gluten feed is a by-product that comes from the wet milling industry or from manufacturers of starch, syrups, oils and sweeteners which make use of corn starch production process. Corn gluten feed is readily available in the USA and is also produced in bulk quantities in India Extraction of residual starch required solubilization of the protein, including breaking of the disulfide bonds. This process altered the gluten structure and properties. Light microscope investigation showed that glutens with residual starch extracted were unable to form fibrillar strands on hydration Gluten is the composite of a gliadin and a glutenin, which is conjoined with starch in the endosperm of various grass-related grains. --> Gluten is the combination of a gliadin and a glutenin. It is found, along with starch, in some grains. Gluten is not starch and starch is not gluten. But they are found together in grain Abstract. A laboratory-scale process is presented for the manufacture of starch and gluten from wheat. Main feature of this process is that whole wheat kernels are crushed dry between smooth rolls prior to wet disintegration in excess water in such way that gluten formation is prevented and fibres can be removed by sieving Wheat Starch. 1. Water, flour mix and gluten separation. Flour is mixed with proportional water, then goes through the homogenizer to three-phase decanter for separation. Streams coming out of decanter are as follows: A starch/fiber, gluten/B starch and animal feed. 2